Pressione sanguigna ambulatoriale e indice di massa ventricolare sinistra nei bambini ipertesi.

Per determinare se la pressione sanguigna ambulatoriale è più predittiva dell’ipertrofia ventricolare sinistra rispetto alla pressione sanguigna occasionale nei bambini ipertesi, sono stati analizzati i dati ecocardiografici e della pressione sanguigna ambulatoriale di 37 bambini ipertesi non trattati. La massa ventricolare sinistra è stata calcolata utilizzando l’equazione di Devereux, l’indice di massa ventricolare sinistro è stato calcolato come massa ventricolare sinistra (in grammi) / altezza (2,7) (in metri) e l’ipertrofia ventricolare sinistra è stata definita come indice di massa ventricolare sinistro> 51 g / m (2.7).

Sono stati calcolati la pressione sanguigna media, il carico di pressione sanguigna e l’indice di pressione sanguigna (pressione sanguigna media divisa per la pressione sanguigna ambulatoriale pediatrica 95 ° percentile). L’indice di massa ventricolare sinistro era fortemente correlato con l’indice della pressione arteriosa sistolica nelle 24 ore (r = 0,43, P = 0,008) ed era anche correlato con la pressione arteriosa sistolica nelle 24 ore (r = 0,34, P = 0,037), sangue sistolico nelle 24 ore carico di pressione (r = 0,38, P = 0,020), carico di pressione sanguigna sistolica sveglia (r = 0,37, P = 0,025), pressione sanguigna sistolica del sonno (r = 0,33, P = 0,048) e carico di pressione sanguigna sistolica del sonno (r = 0,38, P = 0,021).

L’indice di massa ventricolare sinistro non era correlato all’età, al peso, alla pressione sanguigna clinica o alla pressione sanguigna diastolica ambulatoriale. La prevalenza complessiva dell’ipertrofia ventricolare sinistra è stata del 27%. La prevalenza dell’ipertrofia ventricolare sinistra era del 47% (8 su 17) nei pazienti con carico della pressione arteriosa sistolica> 50% e indice della pressione arteriosa sistolica nelle 24 ore> 1,0, rispetto al 10% (2 su 20) nei pazienti senza entrambi i criteri (P = 0,015). Questi dati suggeriscono che il monitoraggio ambulatoriale della pressione arteriosa può essere utile per la valutazione clinica dei bambini ipertesi, identificando quelli ad alto rischio per la presenza di lesioni d’organo.

Un sistema di punteggio clinico per la selezione dei pazienti per il test di mutazione PTEN viene proposto sulla base di uno studio prospettico di 3042 probandi.

 

La sindrome di Cowden (CS) e la sindrome di Bannayan-Riley-Ruvalcaba sono alleliche, definite da mutazioni germinali di PTEN e collettivamente denominate sindrome tumorale da amartoma PTEN. Ad oggi, non ci sono criteri esistenti basati su ampie coorti di pazienti potenziali per selezionare i pazienti per il test di mutazione PTEN. Per affrontare questi problemi, abbiamo condotto uno studio prospettico multicentrico in cui sono stati accumulati 3042 probandi che soddisfacevano i criteri clinici rilassati della CS. La scansione della mutazione PTEN, inclusa l’analisi del promotore e dell’ampia delezione, è stata eseguita per tutti i soggetti.

Mutazioni patogene sono state identificate in 290 individui (9,5%). Per valutare il fenotipo clinico e il genotipo PTEN rispetto all’espressione proteica, abbiamo eseguito immunoblotting (PTEN, P-AKT1, P-MAPK1 / 2) per un sottogruppo di pazienti (n = 423). Al fine di ottenere una stima personalizzata della probabilità pretest di mutazione germinale PTEN, abbiamo sviluppato un modello di pratica clinica ottimizzato per identificare pazienti adulti e pediatrici. Per gli adulti, un punteggio semiquantitativo, il punteggio della Cleveland Clinic (CC), ha prodotto una stima ben calibrata della probabilità pre-test dello stato di PTEN.

Nel complesso, la diminuzione dell’espressione della proteina PTEN è correlata con lo stato di mutazione PTEN; la diminuzione dell’espressione della proteina PTEN era correlata con l’aumento del punteggio CC (p <0,001), ma non con i criteri del National Comprehensive Cancer Network (NCCN) (p = 0,11). Per i pazienti pediatrici, abbiamo identificato criteri altamente sensibili per guidare i test di mutazione PTEN, con caratteristiche fenotipiche distinte dall’ambiente adulto. Il nostro modello ha migliorato la sensibilità e il valore predittivo positivo per la mutazione germinale PTEN rispetto ai criteri NCCN 2010 in entrambe le coorti.

Utilizzo del trasferimento dei compiti per aumentare rapidamente i servizi di trattamento dell’HIV: esperienze da Lusaka, Zambia.

 

L’Organizzazione Mondiale della Sanità sostiene il trasferimento dei compiti, il processo di delega delle funzioni di assistenza clinica da operatori sanitari più specializzati a operatori sanitari meno specializzati, come strategia per raggiungere gli Obiettivi di Sviluppo del Millennio delle Nazioni Unite. Tuttavia, c’è una carenza di letteratura che descrive il trasferimento di compiti nell’Africa subsahariana, dove i servizi per la terapia antiretrovirale (ART) sono aumentati rapidamente di fronte a crisi generalizzate delle risorse umane. Nell’ambito dell’espansione dei servizi ART a Lusaka, Zambia, abbiamo implementato un programma completo di trasferimento delle attività tra i fornitori di servizi sanitari esistenti e i lavoratori della comunità.

La formazione inizia con sessioni didattiche mirate a set di abilità specializzate. Segue un periodo intensivo di tutoraggio pratico, in cui i fornitori vengono associati ai formatori prima di lavorare in modo indipendente. Forniamo una valutazione della qualità continua utilizzando indicatori chiave della qualità dell’assistenza clinica in ogni sito. Le prestazioni del programma vengono riviste trimestralmente con il personale della clinica. Quando vengono identificati i problemi, i membri del personale della clinica progettano e implementano interventi specifici per affrontare aree mirate.

Rabbit PLASMA NS K2 EDTA YG LYO (3mL)
31144L-0	Pel-Freez	10x3ml	EUR 81.31

Rabbit Serum Sterile, trace-hemolized (56-84 days old)
31126-3	Pel-Freez	3L	EUR 694
Description: Produced from whole blood collected from young healthy rabbits (New Zealand White or Californian), 6+ months old. Pooled serum from male and female rabbits that are fasted prior to collection. The serum is processed, bottled, and stored at -20°C. Then the serum was thawed, pooled, passed through a 0.22-micron filter, bottled and returned to -20°C.

TAPI-0
2067-1	Biovision		EUR 398

Antibody analysis - human >30 samples
8005-3	Chondrex	Custom service	EUR 113.8
Description: Antibody analysis - human >30 samples

RealScreen Pediatric FSH ELISA Kit
EL103-096	GenDepot	96T	EUR 1832

Human Plasma Progesterone standard (0 ng/ml)
1955-P4-00	Alpha Diagnostics	10 ml	Ask for price

Haptoglobin, (Phenotype 1-1) Human Plasma
7536-1	Biovision		EUR 321

AGEs-BSA
KH001-A	Sceti		EUR 870
Description: The AGEs-BSA is available in Europe and for worldwide shipping via Gentaur.

Human IgG-RF stripper/Adsorbent (1 ml, sufficient for stripping 50 samples of 100 ul human serum/plasma)
RFS-1	Alpha Diagnostics	1 ml	EUR 103

Rabbit Complement 3-4 WK (1-mL)
31061-0	Pel-Freez	1mL	EUR 93.28

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)
M1039-3	Biovision		EUR 436

LysoPC(14:0/0:0)
HY-113123	MedChemExpress	5mg	EUR 142

pBI121 old Plasmid
PVT3002	Lifescience Market	2 ug	EUR 370

Albumin, Human Plasma
7546-1	Biovision		EUR 240

Plasminogen, Human Plasma
7549-1	Biovision		EUR 283

Prothrombin, Human Plasma
7684-1	Biovision		EUR 180

Ceruloplasmin, Human Plasma
P1452-1	Biovision		EUR 479

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-3	Biovision		EUR 436

Rabbit PLASMA NS EDTA 500mL
31141-1	Pel-Freez	500mL	EUR 261.84

Rabbit PLASMA S EDTA 500mL
31156-1	Pel-Freez	500mL	EUR 285.76

CK PLASMA NS EDTA 500mL
33141-1	Pel-Freez	500mL	EUR 184.63

CK PLASMA NS HEP 500mL
33142-1	Pel-Freez	500mL	EUR 184.63

Bovine PLASMA NS EDTA 500mL
37141-1	Pel-Freez	500mL	EUR 184.63

Bovine PLASMA NS HEP 500mL
37142-1	Pel-Freez	500mL	EUR 184.63

Pig PLASMA NS EDTA 500mL
39541-1	Pel-Freez	500mL	EUR 184.63

Pig PLASMA NS HEP 500mL
39542-1	Pel-Freez	500mL	EUR 184.63

Haptoglobin, Human Plasma (Mixed Type)
7535-1	Biovision		EUR 158

Fibrinogen (plasminogen depleted), Human Plasma
7692-1	Biovision		EUR 294

Antistreptolysin O (ASO), Human Plasma
P1453-1	Biovision		EUR 207

Sheep AGEs ELISA Kit
ESA0009	Abclonal	96Tests	EUR 521

Mouse AGEs ELISA Kit
EMA0009	Abclonal	96Tests	EUR 521

Anserini AGEs ELISA Kit
EAA0009	Abclonal	96Tests	EUR 521

Rabbit AGEs ELISA Kit
ERTA0009	Abclonal	96Tests	EUR 521

Monkey AGEs ELISA Kit
EMKA0009	Abclonal	96Tests	EUR 521

Porcine AGEs ELISA Kit
EPA0009	Abclonal	96Tests	EUR 521

Rat AGEs ELISA Kit
ERA0009	Abclonal	96Tests	EUR 521

Chicken AGEs ELISA Kit
ECKA0009	Abclonal	96Tests	EUR 521

Goat AGEs ELISA Kit
EGTA0009	Abclonal	96Tests	EUR 521

Bovine AGEs ELISA Kit
EBA0009	Abclonal	96Tests	EUR 521

Rabbit BRAIN ACETONE POWDER VARISENS (1-g)
41170-0	Pel-Freez	1g	EUR 104.15

Human IgG1, Myeloma Plasma
20007-G1-1	Alpha Diagnostics	1 mg	EUR 202

Human IgG2, Myeloma Plasma
20007-G2-1	Alpha Diagnostics	1 mg	EUR 202

Human IgG3, Myeloma Plasma
20007-G3-1	Alpha Diagnostics	1 mg	EUR 408

34011-0 Human Serum Off Clot Single Donor (1mL)
34011-1	Pel-Freez	1mL	EUR 68.81

Rabbit PLASMA NS W/ HEPARIN 500mL
31142-1	Pel-Freez	500mL	EUR 261.84

Rabbit PLASMA S NA CIT 500mL
31155-1	Pel-Freez	500mL	EUR 285.76

CK PLASMA NS S-CIT 500mL
33140-1	Pel-Freez	500mL	EUR 184.63

Bovine PLASMA NS S-CIT 500mL
37140-1	Pel-Freez	500mL	EUR 184.63

Pig PLASMA NS S-CIT 500mL
39540-1	Pel-Freez	500mL	EUR 184.63

Haptoglobin, (Phenotype 2-2) Human Plasma
7537-1	Biovision		EUR 321

Alpha 2 HS Glycoprotein, Human Plasma
7548-1	Biovision		EUR 272

Thrombin, Active, Bovine Plasma (Technical grade)
7591-1	Biovision		EUR 120

Thrombin, Active, Bovine Plasma (High Activity)
7592-1	Biovision		EUR 158

Lung Lysate (14 Days Old)
1402-14	ProSci	0.1 mg	EUR 191
Description: Lung tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Lung Lysate (7 Days Old)
1402-7	ProSci	0.1 mg	EUR 191
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (14 Days Old)
1403-14	ProSci	0.1 mg	EUR 191
Description: Brain tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)
1403-7	ProSci	0.1 mg	EUR 191
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (14 Days Old)
1404-14	ProSci	0.1 mg	EUR 191
Description: Liver tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)
1404-7	ProSci	0.1 mg	EUR 191
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (14 Days Old)
1405-14	ProSci	0.1 mg	EUR 191
Description: Kidney tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)
1405-7	ProSci	0.1 mg	EUR 191
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (14 Days Old)
1406-14	ProSci	0.1 mg	EUR 191
Description: Spleen tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)
1406-7	ProSci	0.1 mg	EUR 191
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (14 Days Old)
1409-14	ProSci	0.1 mg	EUR 191
Description: Thymus tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)
1409-7	ProSci	0.1 mg	EUR 191
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (14 Day Old)
1415-14	ProSci	0.1 mg	EUR 191
Description: Stomach tissue lysate (14 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (7 Day Old)
1415-7	ProSci	0.1 mg	EUR 191
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (14 Days Old)
1419-14	ProSci	0.1 mg	EUR 191
Description: Skin tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)
1419-7	ProSci	0.1 mg	EUR 191
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (14 Days Old)
1420-14	ProSci	0.1 mg	EUR 191
Description: Eye tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)
1420-7	ProSci	0.1 mg	EUR 191
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Heart Lysate (14 Days Old)
1401-14	ProSci	0.1 mg	EUR 191
Description: Heart tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Yersinia Enterocolitica 0:3 Protein
abx061492-01ml	Abbexa	0.1 ml	EUR 551

Rabbit PLASMA NS NA CIT Y 500mL
31140-1	Pel-Freez	500mL	EUR 261.84

Rabbit PLASMA NS K2 EDTA Y 500mL
31144-1	Pel-Freez	500mL	EUR 261.84

Rabbit PLASMA NS K3 EDTA YG 500mL
31145-1	Pel-Freez	500mL	EUR 233.56

Guinea Pig AGEs ELISA Kit
EGA0009	Abclonal	96Tests	EUR 521

Mouse Aquaporin 0(AQP-0)ELISA Kit
GA-E0524MS-48T	GenAsia Biotech	48T	EUR 336

Mouse Aquaporin 0(AQP-0)ELISA Kit
GA-E0524MS-96T	GenAsia Biotech	96T	EUR 534

Rat Aquaporin 0(AQP-0)ELISA Kit
GA-E0572RT-48T	GenAsia Biotech	48T	EUR 336

Rat Aquaporin 0(AQP-0)ELISA Kit
GA-E0572RT-96T	GenAsia Biotech	96T	EUR 534

Human Aquaporin 0(AQP-0)ELISA Kit
GA-E1301HM-48T	GenAsia Biotech	48T	EUR 289

Human Aquaporin 0(AQP-0)ELISA Kit
GA-E1301HM-96T	GenAsia Biotech	96T	EUR 466

Human Aquaporin 0,AQP-0 ELISA Kit
201-12-1285	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Rat Aquaporin 0(AQP-0)ELISA Kit
QY-E11080	Qayee Biotechnology	96T	EUR 361

Mouse Aquaporin 0(AQP-0)ELISA Kit
QY-E20161	Qayee Biotechnology	96T	EUR 361

Human Aquaporin 0(AQP-0)ELISA Kit
QY-E01463	Qayee Biotechnology	96T	EUR 361

Rat Aquaporin 0,AQP-0 ELISA Kit
CN-01885R1	ChemNorm	96T	EUR 471

Rat Aquaporin 0,AQP-0 ELISA Kit
CN-01885R2	ChemNorm	48T	EUR 322

Mouse Aquaporin 0,AQP-0 ELISA Kit
CN-02762M1	ChemNorm	96T	EUR 464

Mouse Aquaporin 0,AQP-0 ELISA Kit
CN-02762M2	ChemNorm	48T	EUR 313

Human Aquaporin 0,AQP-0 ELISA Kit
CN-04417H1	ChemNorm	96T	EUR 464

Human Aquaporin 0,AQP-0 ELISA Kit
CN-04417H2	ChemNorm	48T	EUR 313

Rat Aquaporin 0,AQP-0 ELISA kit
E02A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Aquaporin 0,AQP-0 ELISA kit
E02A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Aquaporin 0,AQP-0 ELISA kit
E02A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Aquaporin 0,AQP-0 ELISA kit
E08A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Aquaporin 0,AQP-0 ELISA kit
E08A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Aquaporin 0,AQP-0 ELISA kit
E08A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Aquaporin 0,AQP-0 ELISA kit
E09A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Aquaporin 0,AQP-0 ELISA kit
E09A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Aquaporin 0,AQP-0 ELISA kit
E09A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Aquaporin 0,AQP-0 ELISA kit
E01A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Human Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Aquaporin 0,AQP-0 ELISA kit
E01A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Human Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Aquaporin 0,AQP-0 ELISA kit
E01A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Human Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Aquaporin 0,AQP-0 ELISA kit
E04A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rabbit Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Aquaporin 0,AQP-0 ELISA kit
E04A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Rabbit Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Aquaporin 0,AQP-0 ELISA kit
E04A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Rabbit Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Aquaporin 0,AQP-0 ELISA kit
E03A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Aquaporin 0,AQP-0 ELISA kit
E03A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Aquaporin 0,AQP-0 ELISA kit
E03A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Aquaporin 0,AQP-0 ELISA kit
E07A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Porcine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Aquaporin 0,AQP-0 ELISA kit
E07A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Porcine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Aquaporin 0,AQP-0 ELISA kit
E07A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Porcine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Aquaporin 0,AQP-0 ELISA kit
E06A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Goat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Aquaporin 0,AQP-0 ELISA kit
E06A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Goat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Aquaporin 0,AQP-0 ELISA kit
E06A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Goat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Heart Lysate (7 day old mouse)
1401-7	ProSci	0.1 mg	EUR 191
Description: Heart tissue lysate (7 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

SmartSEC HT EV Isolation System (for Serum & Plasma)
SSEC096A-1	SBI	96 rxn	EUR 2219

Western Blot Box - 2 7/8 x 1 3/16 x 3/4in.; 7.3 x 3 x 1.9cm
B1200-3	MTC Bio	5/pack	EUR 63.77
Description: Western Blot Boxes

ELISA kit for Mouse AQP-0 (Aquaporin 0)
E-EL-M0144	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse AQP-0 (Aquaporin 0) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Aquaporin 0 (AQP-0)
KTE61608-48T	Abbkine	48T	EUR 354
Description: Quantitative sandwich ELISA for measuring Human Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Aquaporin 0 (AQP-0)
KTE61608-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Aquaporin 0 (AQP-0)
KTE61608-96T	Abbkine	96T	EUR 572
Description: Quantitative sandwich ELISA for measuring Human Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Aquaporin 0 (AQP-0)
KTE100611-48T	Abbkine	48T	EUR 354
Description: Quantitative sandwich ELISA for measuring Rat Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Aquaporin 0 (AQP-0)
KTE100611-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2252
Description: Quantitative sandwich ELISA for measuring Rat Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Aquaporin 0 (AQP-0)
KTE100611-96T	Abbkine	96T	EUR 572
Description: Quantitative sandwich ELISA for measuring Rat Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Guinea pig Aquaporin 0,AQP-0 ELISA kit
E05A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Guinea pig Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Aquaporin 0,AQP-0 ELISA kit
E05A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Guinea pig Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Aquaporin 0,AQP-0 ELISA kit
E05A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Guinea pig Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

2019-nCoV IgG/IgM RapiCard InstaTest for Whole Blood/Serum/Plasma samples
GEN-176552-25tests	Cortez Diagnostic	25 tests	EUR 276
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

2019-nCoV IgG/IgM RapiCard InstaTest for Whole Blood/Serum/Plasma samples
GEN-176552-50tests	Cortez Diagnostic	50 tests	EUR 472
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

SP2/0-Ag14
C0033005	Addexbio	One Frozen vial	EUR 455

0-Trufluoromethylboronic Acid
20-abx182800	Abbexa	EUR 328.00 EUR 203.00 EUR 175.00	100 g 25 g 5 g

Aquaporin 0 antibody
70R-50059	Fitzgerald	100 ul	EUR 244
Description: Purified Polyclonal Aquaporin 0 antibody

1961358-02-0
TBW00994	ChemNorm	10mg	Ask for price

90685-26-0
TBW01004	ChemNorm	10mg	Ask for price

151135-83-0
TBZ0275	ChemNorm	unit	Ask for price

180161-88-0
TBZ0379	ChemNorm	unit	Ask for price

109237-05-0
TBZ0434	ChemNorm	unit	Ask for price

133921-57-0
TBZ0598	ChemNorm	unit	Ask for price

136014-41-0
TBZ0604	ChemNorm	unit	Ask for price

122564-89-0
TBZ0684	ChemNorm	unit	Ask for price

164596-57-0
TBZ0794	ChemNorm	unit	Ask for price

109269-99-0
TBZ0998	ChemNorm	unit	Ask for price

156006-37-0
TBZ1078	ChemNorm	unit	Ask for price

199922-56-0
TBZ1251	ChemNorm	unit	Ask for price

173293-92-0
TBZ30028	ChemNorm	unit	Ask for price

Mini Pig (Goettingen) Plasma control (non-immunized) Mixed breed
MPIG-1	Alpha Diagnostics	1 ml	EUR 225

ExoQuick PLUS Exosome Purification Kit - for serum and plasma
EQPL10A-1	SBI	10 reactions	EUR 579

Thymus Dissociation System 3 (Epithelial), Rat, less than 6wk old
4-20443	CHI Scientific	ea	Ask for price

Human Plasma Progesterone standard (100 ng/ml)
1955-P4-1	Alpha Diagnostics	1 ml	Ask for price

Human IgA purified (native, Plasma) (isotype control)
20007-5-1	Alpha Diagnostics	1 mg	EUR 164

Dal 2005 al 2007, abbiamo formato 516 operatori sanitari nel trattamento dell’HIV negli adulti; 270 nel trattamento dell’HIV pediatrico; 341 in consulenza per l’adesione; 91 in un corso di “triage” per infermiere specializzato e 93 in un programma intensivo di tutoraggio clinico. La valutazione della qualità in corso ha dimostrato un miglioramento tra gli indicatori di qualità dell’assistenza clinica, nonostante i volumi di pazienti in rapida crescita. La nostra strategia di spostamento delle attività è stata progettata per soddisfare le attuali esigenze degli operatori sanitari e per sostenere le attività di scale-up ART. Sebbene questo approccio abbia avuto successo, sono urgentemente necessarie anche soluzioni a lungo termine alla crisi delle risorse umane per espandere il numero di fornitori e rallentare la migrazione del personale fuori dalla regione.