Iperuricemia nell’ipertensione primaria infantile.

 

Modelli animali sperimentali suggeriscono che l’acido urico potrebbe avere un ruolo patogeno nello sviluppo precoce dell’ipertensione primaria. Abbiamo ipotizzato che l’acido urico sierico sia correlato alla pressione sanguigna nei bambini con ipertensione primaria di nuova insorgenza, non trattata. Abbiamo valutato 125 bambini consecutivi inviati al programma renale pediatrico Baylor per la valutazione dell’ipertensione. Nessuno dei soggetti era stato precedentemente valutato o trattato per l’ipertensione. I bambini avevano un’età compresa tra 6 e 18 anni (media, 13,4 +/- 3,3) e avevano una normale funzione renale (clearance della creatinina> 80 mL x min (-1) x 1,73 m (-2)).

Sessantatré bambini avevano ipertensione primaria, 40 avevano ipertensione secondaria e 22 avevano ipertensione da camice bianco. Quaranta controlli con pressione sanguigna normale sono stati reclutati dalla clinica renale. I livelli di acido urico erano direttamente correlati con la pressione sanguigna sistolica (r = 0,80, P = 0,0002) e diastolica (r = 0,66, P = 0,0006) nei controlli e nei soggetti con ipertensione primaria ed erano indipendenti dalla funzione renale. Concentrazioni sieriche di acido urico> 5,5 mg / dL sono state trovate nell’89% dei soggetti con ipertensione primaria, nel 30% con ipertensione secondaria, nello 0% con ipertensione da camice bianco e nello 0% dei controlli.

Concludiamo che l’acido urico sierico è direttamente correlato alla pressione sanguigna nei bambini non trattati e che un valore di acido urico sierico> 5,5 mg / dL in un adolescente valutato per l’ipertensione suggerisce fortemente l’ipertensione primaria rispetto all’ipertensione da camice bianco o secondaria. Questi risultati sono coerenti con l’ipotesi che l’acido urico possa avere un ruolo nella patogenesi precoce dell’ipertensione primaria.

Fourteen questions from the Kiddie Schedule for Affective Disorders and Schizophrenia for School-Age Children-Present Episode (K-SADS-P) 1986 version plus a new item assessing mood lability were used to construct a clinician-rated mania rating scale (K-SADS-MRS). Interrater reliability was determined prospectively with 22 patients from a bipolar outpatient clinic. Sensitivity to treatment effects was determined in a separate cohort of 23 patients.

Valutazione funzionale dei pazienti pediatrici con dolore: proprietà psicometriche dell’inventario della disabilità funzionale.

 

L’inventario della disabilità funzionale (FDI; Walker LS, Greene JW. L’inventario della disabilità funzionale: misurazione di una dimensione trascurata dello stato di salute del bambino. J Pediatr Psychol 1991; 16: 39-58) valuta le limitazioni dell’attività in bambini e adolescenti con una varietà di pediatria condizioni. Questo studio ha valutato le proprietà psicometriche dell’FDI nei pazienti pediatrici con dolore. I partecipanti includevano 596 pazienti con dolore addominale cronico, di età compresa tra 8 e 17 anni, e un sottogruppo dei loro genitori (n = 151) che hanno completato l’FDI e le misure di dolore, limitazioni nelle attività scolastiche e sintomi somatici e depressivi durante una visita clinica.

L’affidabilità del test-retest era alta a 2 settimane (rapporto del bambino, .74; rapporto del genitore, .64) e moderato a 3 mesi (rapporto del bambino, .48; rapporto del genitore, .39). L’affidabilità della coerenza interna è stata eccellente, con un range da .86 a .91. La validità è stata supportata da correlazioni significative dei punteggi FDI riportati da genitori e figli con misure di disabilità, dolore e sintomi somatici legati alla scuola. I risultati dello studio si aggiungono a un corpo crescente di letteratura empirica a supporto dell’affidabilità e della validità dell’FDI per la valutazione funzionale dei pazienti pediatrici con dolore cronico.

L’esposizione alle spore disperse nell’aria della muffa comune Alternaria alternata è stata implicata negli attacchi di asma. Tale esposizione è particolarmente frequente nel Midwest durante i mesi estivi e autunnali. Per determinare il ruolo di A. alternata nell’innescare gravi attacchi di asma, abbiamo studiato i casi di 11 pazienti con asma che avevano avuto un arresto respiratorio improvviso e determinato la frequenza di sensibilità a questo allergene in questi pazienti.

Consegna genica sistemica in specie di grandi dimensioni per il targeting del midollo spinale, del cervello e dei tessuti periferici per i disturbi pediatrici.

 

Il virus adeno-associato di tipo 9 (AAV9) è un potente strumento per il rilascio di geni in tutto il sistema nervoso centrale (SNC) dopo l’iniezione endovenosa. I risultati preclinici nei modelli pediatrici di atrofia muscolare spinale (SMA) e disturbi da accumulo lisosomiale forniscono un caso convincente per portare l’AAV9 alla clinica. Un passo importante della traduzione è dimostrare un efficiente targeting del sistema nervoso centrale in animali di grandi dimensioni di varie età. Nel presente studio, abbiamo testato AAV9 iniettato sistemicamente in macachi cynomolgus, somministrato alla nascita fino ai 3 anni di età per il targeting del sistema nervoso centrale e dei tessuti periferici.

Mostriamo che AAV9 era efficiente nell’attraversare la barriera emato-encefalica (BBB) ​​in tutti i punti temporali studiati. L’espressione del transgene è stata rilevata principalmente nelle cellule gliali in tutto il cervello, nei neuroni dei gangli della radice dorsale e nei neuroni motori all’interno del midollo spinale, fornendo fiducia per la traduzione ai pazienti SMA. L’iniezione sistemica ha anche mirato efficacemente ai muscoli scheletrici e agli organi periferici.

Eye Lysate (0 Days Old)
1420-0	ProSci	0.1 mg	EUR 191
Description: Eye tissue lysate (0 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Heart Lysate (0 day old mouse)
1401-0	ProSci	0.1 mg	EUR 191
Description: Heart tissue lysate (0 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Human Fecal calprotectin(fecal calprotectin) ELISA Kit
QY-E05394	Qayee Biotechnology	96T	EUR 361

Rabbit Serum Sterile, trace-hemolized (56-84 days old)
31126-3	Pel-Freez	3L	EUR 694
Description: Produced from whole blood collected from young healthy rabbits (New Zealand White or Californian), 6+ months old. Pooled serum from male and female rabbits that are fasted prior to collection. The serum is processed, bottled, and stored at -20°C. Then the serum was thawed, pooled, passed through a 0.22-micron filter, bottled and returned to -20°C.

TAPI-0
2067-1	Biovision		EUR 398

Antibody analysis - human >30 samples
8005-3	Chondrex	Custom service	EUR 113.8
Description: Antibody analysis - human >30 samples

RealScreen Pediatric FSH ELISA Kit
EL103-096	GenDepot	96T	EUR 1832

ISOLATE Fecal DNA Kit
BIO-52038	Bioline	100 preps (Kit 2 x 50 preps)	Ask for price

ISOLATE Fecal DNA Kit
BIO-52082	Bioline	50 preps	Ask for price

AGEs-BSA
KH001-A	Sceti		EUR 870
Description: The AGEs-BSA is available in Europe and for worldwide shipping via Gentaur.

Rabbit Complement 3-4 WK (1-mL)
31061-0	Pel-Freez	1mL	EUR 93.28

LysoPC(14:0/0:0)
HY-113123	MedChemExpress	5mg	EUR 142

pBI121 old Plasmid
PVT3002	Lifescience Market	2 ug	EUR 370

Hemoglobin Fecal Occult Blood (FOB) Antibody
abx024027-1mg	Abbexa	1 mg	EUR 829

Hemoglobin Fecal Occult Blood (FOB) Antibody
abx024028-1mg	Abbexa	1 mg	EUR 829

Sheep AGEs ELISA Kit
ESA0009	Abclonal	96Tests	EUR 521

Mouse AGEs ELISA Kit
EMA0009	Abclonal	96Tests	EUR 521

Monkey AGEs ELISA Kit
EMKA0009	Abclonal	96Tests	EUR 521

Porcine AGEs ELISA Kit
EPA0009	Abclonal	96Tests	EUR 521

Rat AGEs ELISA Kit
ERA0009	Abclonal	96Tests	EUR 521

Rabbit AGEs ELISA Kit
ERTA0009	Abclonal	96Tests	EUR 521

Anserini AGEs ELISA Kit
EAA0009	Abclonal	96Tests	EUR 521

Goat AGEs ELISA Kit
EGTA0009	Abclonal	96Tests	EUR 521

Chicken AGEs ELISA Kit
ECKA0009	Abclonal	96Tests	EUR 521

Bovine AGEs ELISA Kit
EBA0009	Abclonal	96Tests	EUR 521

34011-0 Human Serum Off Clot Single Donor (1mL)
34011-1	Pel-Freez	1mL	EUR 68.81

Rabbit BRAIN ACETONE POWDER VARISENS (1-g)
41170-0	Pel-Freez	1g	EUR 104.15

Heart Lysate (14 Days Old)
1401-14	ProSci	0.1 mg	EUR 191
Description: Heart tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Lung Lysate (14 Days Old)
1402-14	ProSci	0.1 mg	EUR 191
Description: Lung tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Lung Lysate (7 Days Old)
1402-7	ProSci	0.1 mg	EUR 191
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (14 Days Old)
1403-14	ProSci	0.1 mg	EUR 191
Description: Brain tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)
1403-7	ProSci	0.1 mg	EUR 191
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (14 Days Old)
1404-14	ProSci	0.1 mg	EUR 191
Description: Liver tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)
1404-7	ProSci	0.1 mg	EUR 191
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (14 Days Old)
1405-14	ProSci	0.1 mg	EUR 191
Description: Kidney tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)
1405-7	ProSci	0.1 mg	EUR 191
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (14 Days Old)
1406-14	ProSci	0.1 mg	EUR 191
Description: Spleen tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)
1406-7	ProSci	0.1 mg	EUR 191
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (14 Days Old)
1409-14	ProSci	0.1 mg	EUR 191
Description: Thymus tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)
1409-7	ProSci	0.1 mg	EUR 191
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (14 Day Old)
1415-14	ProSci	0.1 mg	EUR 191
Description: Stomach tissue lysate (14 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (7 Day Old)
1415-7	ProSci	0.1 mg	EUR 191
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (14 Days Old)
1419-14	ProSci	0.1 mg	EUR 191
Description: Skin tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)
1419-7	ProSci	0.1 mg	EUR 191
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (14 Days Old)
1420-14	ProSci	0.1 mg	EUR 191
Description: Eye tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)
1420-7	ProSci	0.1 mg	EUR 191
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Yersinia Enterocolitica 0:3 Protein
abx061492-01ml	Abbexa	0.1 ml	EUR 551

Guinea Pig AGEs ELISA Kit
EGA0009	Abclonal	96Tests	EUR 521

Mouse Aquaporin 0(AQP-0)ELISA Kit
GA-E0524MS-48T	GenAsia Biotech	48T	EUR 336

Mouse Aquaporin 0(AQP-0)ELISA Kit
GA-E0524MS-96T	GenAsia Biotech	96T	EUR 534

Rat Aquaporin 0(AQP-0)ELISA Kit
GA-E0572RT-48T	GenAsia Biotech	48T	EUR 336

Rat Aquaporin 0(AQP-0)ELISA Kit
GA-E0572RT-96T	GenAsia Biotech	96T	EUR 534

Human Aquaporin 0(AQP-0)ELISA Kit
GA-E1301HM-48T	GenAsia Biotech	48T	EUR 289

Human Aquaporin 0(AQP-0)ELISA Kit
GA-E1301HM-96T	GenAsia Biotech	96T	EUR 466

Human Aquaporin 0,AQP-0 ELISA Kit
201-12-1285	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Rat Aquaporin 0(AQP-0)ELISA Kit
QY-E11080	Qayee Biotechnology	96T	EUR 361

Mouse Aquaporin 0(AQP-0)ELISA Kit
QY-E20161	Qayee Biotechnology	96T	EUR 361

Human Aquaporin 0(AQP-0)ELISA Kit
QY-E01463	Qayee Biotechnology	96T	EUR 361

Rat Aquaporin 0,AQP-0 ELISA Kit
CN-01885R1	ChemNorm	96T	EUR 471

Rat Aquaporin 0,AQP-0 ELISA Kit
CN-01885R2	ChemNorm	48T	EUR 322

Mouse Aquaporin 0,AQP-0 ELISA Kit
CN-02762M1	ChemNorm	96T	EUR 464

Mouse Aquaporin 0,AQP-0 ELISA Kit
CN-02762M2	ChemNorm	48T	EUR 313

Human Aquaporin 0,AQP-0 ELISA Kit
CN-04417H1	ChemNorm	96T	EUR 464

Human Aquaporin 0,AQP-0 ELISA Kit
CN-04417H2	ChemNorm	48T	EUR 313

Rat Aquaporin 0,AQP-0 ELISA kit
E02A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Aquaporin 0,AQP-0 ELISA kit
E02A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Aquaporin 0,AQP-0 ELISA kit
E02A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Aquaporin 0,AQP-0 ELISA kit
E08A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Aquaporin 0,AQP-0 ELISA kit
E08A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Aquaporin 0,AQP-0 ELISA kit
E08A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Aquaporin 0,AQP-0 ELISA kit
E04A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rabbit Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Aquaporin 0,AQP-0 ELISA kit
E04A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Rabbit Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Aquaporin 0,AQP-0 ELISA kit
E04A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Rabbit Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Aquaporin 0,AQP-0 ELISA kit
E09A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Aquaporin 0,AQP-0 ELISA kit
E09A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Aquaporin 0,AQP-0 ELISA kit
E09A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Aquaporin 0,AQP-0 ELISA kit
E03A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Aquaporin 0,AQP-0 ELISA kit
E03A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Aquaporin 0,AQP-0 ELISA kit
E03A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Aquaporin 0,AQP-0 ELISA kit
E07A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Porcine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Aquaporin 0,AQP-0 ELISA kit
E07A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Porcine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Aquaporin 0,AQP-0 ELISA kit
E07A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Porcine Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Aquaporin 0,AQP-0 ELISA kit
E06A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Goat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Aquaporin 0,AQP-0 ELISA kit
E06A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Goat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Aquaporin 0,AQP-0 ELISA kit
E06A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Goat Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Aquaporin 0,AQP-0 ELISA kit
E01A1918-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Human Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Aquaporin 0,AQP-0 ELISA kit
E01A1918-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Human Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Aquaporin 0,AQP-0 ELISA kit
E01A1918-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Human Aquaporin 0,AQP-0 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Heart Lysate (7 day old mouse)
1401-7	ProSci	0.1 mg	EUR 191
Description: Heart tissue lysate (7 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

ELISA kit for Mouse AQP-0 (Aquaporin 0)
E-EL-M0144	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse AQP-0 (Aquaporin 0) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Aquaporin 0 (AQP-0)
KTE61608-48T	Abbkine	48T	EUR 354
Description: Quantitative sandwich ELISA for measuring Human Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Aquaporin 0 (AQP-0)
KTE61608-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Aquaporin 0 (AQP-0)
KTE61608-96T	Abbkine	96T	EUR 572
Description: Quantitative sandwich ELISA for measuring Human Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Aquaporin 0 (AQP-0)
KTE100611-48T	Abbkine	48T	EUR 354
Description: Quantitative sandwich ELISA for measuring Rat Aquaporin 0 (AQP-0) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Per mirare specificamente al sistema nervoso centrale, abbiamo esplorato la consegna di AAV9 al liquido cerebrospinale (CSF). L’iniezione di CSF ha mirato efficacemente ai motoneuroni e ha limitato l’espressione genica al sistema nervoso centrale, fornendo un percorso di consegna alternativo e requisiti di produzione potenzialmente inferiori per i pazienti più grandi e più grandi. I nostri risultati supportano l’uso di AAV9 per il trasferimento genico al sistema nervoso centrale per i disturbi nelle popolazioni pediatriche.